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KMID : 0391319930030010039
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Korean Journal of Biological Response Modifiers
1993 Volume.3 No. 1 p.39 ~ p.46
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Effects of Granulocyte-Macrophage Colony-Stimulating Factor and Granulocyte Colony-Stimulating Factor on Colony Formation of Human Cancer Cell Lines
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홍원선
김창민/강윤구/임영혁/이춘택/김유척/이진오/강태웅
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Abstract
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Stimulatory effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF) on the colony formation were investigated using human tumor colony assay in seven human cancer cell lines, two
sublines
resistant to adriamycin and two sublines resistant to cisplatin: PC-9 (pulmonary adenocarcinoma), PC-14 (pulmonary adenocarcinoma), MKN-45 (gastric adenocarcinoma0, KATO III 9gastric adenocarcinoma0, K-562 (chronic myelogenous leukemia0, COLO 205
(colon
adenocarcinma), MKN/ADM (subline of MKN-45 resistant to adriamycn), PC-CDDP (subline of PC-14) resistant to cisplatin) and MKN/CDDP (subline of MKN-45 resistant of PC-14 resistant to cisplatin). Cancer cells were plated at concentrations
(cells/well) of
1×10E3 and 1×10E5 in the upper layer of the two-layer agar culture system. Two kinds of recombinant human GM-CSF (LBD-005 and CSF 39-300) and two kinds of recombinant human G-CSF (Grasin and Neutrogin) were tested by the additon of final
concentrations of 0.01, 0.1 and 1.0㎍/ml of GM-CSF and G-CSF to the lower layer to allow the continuous exposure of cancer cells to GM-CSF and G-CSF for seven and 14 days. Colony formations (%) of 11 cell lines treated with GM-CSF and G-CSF were
85-113%
and 87-109% at the concentrations of 1×10E3 and 1×10E5 plated, respectively, after the incubation of 14 days, which were not significantly different from those of control wells. These results suggest that GM-CSF and G CSF, in all of the cell
lines
tested in this study, do not directly stimulate the growth of cancer cells.
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KEYWORD
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